polyclonal antibodies against adipor1  (Bioss)

Journal: PLoS ONE

Article Title: The impact of obesity and adiponectin signaling in patients with renal cell carcinoma: A potential mechanism for the “obesity paradox”

doi: 10.1371/journal.pone.0171615

Figure Lengend Snippet: The mRNA levels of adiponectin receptors in tissues extracted from patients with RCC who underwent renal surgery.

Article Snippet: The tissues were incubated with a rabbit polyclonal primary antibody against AdipoR1 (Abbiotec, San Diego, CA) diluted 1:200.

Techniques: Expressing

Journal: PLoS ONE

Article Title: The impact of obesity and adiponectin signaling in patients with renal cell carcinoma: A potential mechanism for the “obesity paradox”

doi: 10.1371/journal.pone.0171615

Figure Lengend Snippet: An immunoreactivity score > 4 was defined as high AdipoR1 levels, and an immunoreactivity score ≤4 was defined as low AdipoR1 levels. A Representative images of low and high AdipoR1 levels in human RCC epithelial tissue. AdipoR1 immunoreactivity score was not significantly associated with CSS in the overall RCC patient population (B) and in patients with non-metastatic RCC (C).

Article Snippet: The tissues were incubated with a rabbit polyclonal primary antibody against AdipoR1 (Abbiotec, San Diego, CA) diluted 1:200.

Techniques:

Journal: PLoS ONE

Article Title: The impact of obesity and adiponectin signaling in patients with renal cell carcinoma: A potential mechanism for the “obesity paradox”

doi: 10.1371/journal.pone.0171615

Figure Lengend Snippet: The relationship between AdipoR1 expression and clinicopathological outcomes by immunohistochemistry.

Article Snippet: The tissues were incubated with a rabbit polyclonal primary antibody against AdipoR1 (Abbiotec, San Diego, CA) diluted 1:200.

Techniques: Expressing, Immunohistochemistry

Journal: PLoS ONE

Article Title: The impact of obesity and adiponectin signaling in patients with renal cell carcinoma: A potential mechanism for the “obesity paradox”

doi: 10.1371/journal.pone.0171615

Figure Lengend Snippet: A. Adiponectin receptor 1 (AdipoR1) (upper) and β-actin (lower) protein levels in Caki-1, Caki-2, 786-O, ACHN, and RENCA cells were determined using western blot. AdipoR1 was detected in all cell lines. B. Caki-2 and 786-O cells were treated with various concentrations of recombinant adiponectin (Ad) (25–1000 ng/mL) and analyzed using an MTT assay 0, 24, and 48 h after adiponectin stimulation. *p < 0.05 compared with cell viability at 0 h. C. The histogram presents the invasive capacity of Caki-2 and 786-O cells treated with 250 or 1000 ng/ml recombinant adiponectin. The number of invasive cells in adiponectin-treated cells was compared with the number of invasive cells at 0 h (untreated cells). D. Caki-2 and 786-O cells were stimulated with 250–1000 ng/ml recombinant adiponectin. Cell migration to the wound surface was analyzed under a light microscope. The diameter of the wound in Caki-2 and 786-O cells treated with various concentration of recombinant adiponectin for 24 h was compared with the diameter of the wound in untreated cells.

Article Snippet: The tissues were incubated with a rabbit polyclonal primary antibody against AdipoR1 (Abbiotec, San Diego, CA) diluted 1:200.

Techniques: Western Blot, Recombinant, MTT Assay, Migration, Light Microscopy, Concentration Assay

Journal: Environmental Health and Preventive Medicine

Article Title: Simultaneous changes in high-fat and high-cholesterol diet-induced steatohepatitis and severe fibrosis and those underlying molecular mechanisms in novel SHRSP5/Dmcr rat

doi: 10.1007/s12199-012-0273-y

Figure Lengend Snippet: Effects of HFC-diet treatment on hepatic adiponectin receptor 1 and 2 (AdipoR1, AdipoR2), 5′-adenosine monophosphate-activated protein kinase-α subunit ½ (AMPKα1/2) and phosphorylated AMPKα (p-AMPKα) protein levels and mRNA expressions. a Western blot results of respective protein expressions in whole liver tissue homogenates. b, c Hepatic AdipoR1 protein (b) and mRNA expression (c) ratios. d, e Hepatic AdipoR2 protein (d) and mRNA expression (e) ratios. f, g AMPKα1/2 (f) and p-AMPKα (g) protein expression ratios. Each histogram represents a mean ratio ± SD. *p < 0.05, **p < 0.01 compared with the SP-diet group within each diet–treatment period

Article Snippet: The primary polyclonal antibodies against adiponectin receptor 1 (AdipoR1) and 2 (AdipoR2) (Alpha Diagnostic International, San Antonio, TX), or α-smooth muscle actin (α-SMA) (Sigma Aldrich) were purchased from the source shown herein.

Techniques: Western Blot, Expressing